Which statement is false? In CRISPR-cas system the gRNA guide the cas protein to
ID: 177215 • Letter: W
Question
Which statement is false? In CRISPR-cas system the gRNA guide the cas protein to the exact target sequence and then cas cleavage site will cut the target DNA on both strands. T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. In nature, the repeat-spacer segment of the CRISPR-cas defense system in bacteria, can be added to the cell memory every time cell is attacked by a new virus. This addition is through incorporation of viral sequence recognition site to the bacterial chromosome where other repeat-spacers are located In a knock-out mouse the gene is expressed into mRNA but a mechanism prevents the process that translates m RNA into protein.Explanation / Answer
A is the right answer.
The Cas9 nickase generated by mutating only one nuclease domain can only cleave one strand of the target DNA, which creates a nick thought to be repaired efficiently in cells.
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