You become a Crime Scene Investigator and are analyzing one blood sample that ha

Question

You become a Crime Scene Investigator and are analyzing one blood sample that has been obtained from an actual crime scene. You are using two SSLP markers (M1 and M2) for genotyping the blood sample. M1 is located in human chromosome 19 and M2 in Y chromosome. The results of your genotyping are shown as below:

PCR and gel electrophoresis (GE) of M1 SSLP products show seven different sizes of DNA fragments (210, 220, 230, 240, 250, 260, 270 bp in length)

PCR and GE of M2 SSLP products show three different sizes of DNA fragments (150, 160, 170 bp in length)

1. Based on the results shown above, you need to provide information regarding how many people’s DNA have been mixed in this blood sample. What is the minimum number of people for this mixed blood sample?

Explanation / Answer

Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.

Human Chromosome: human body is formed through chromosomes, chromose contains all the dna,rna and other components that are neccessary for the development of the 'feotus'.

Simple Sequence Length Polymorphisms (SSLPs) are used as genetic markers with Polymerase Chain Reaction (PCR). An SSLP is a type of polymorphism: a difference in DNA sequence amongst individuals. SSLPs are repeated sequences over varying base lengths in intergenic regions of deoxyribonucleic acid (DNA).

XX refers to an Person to be Female
XY refers to be an male

Chromosome 19 has the highest gene density of all human chromosomes, more than double the genome-wide average. The large clustered gene families, corresponding high G + C content, CpG islands and density of repetitive DNA indicate a chromosome rich in biological and evolutionary significance. Here we describe 55.8 million base pairs of highly accurate finished sequence representing 99.9% of the euchromatin portion of the chromosome. Manual curation of gene loci reveals 1,461 protein-coding genes and 321 pseudogenes. Among these are genes directly implicated in mendelian disorders, including familial hypercholesterolaemia and insulin-resistant diabetes. Nearly one-quarter of these genes belong to tandemly arranged families, encompassing more than 25% of the chromosome. Comparative analyses show a fascinating picture of conservation and divergence, revealing large blocks of gene orthology with rodents, scattered regions with more recent gene family expansions and deletions, and segments of coding and non-coding conservation with the distant fish species Takifugu.

They are about 3 people blood that is been founded. In that one is male and the remaining two are females.

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