I Need to answer T or F I already know the anwers I need help with why they are

Question

I Need to answer T or F I already know the anwers I need help with why they are false ?

T F Mismatch repair in E. coli involves a process whereby a base mismatch created by a replication error is recognized by MutL and Dam proteins because it can recognize hemimethylated DNA and then the error is removed by excision of part of the template strand via host 5’ or 3’ exonucleases and then resynthesized by DNA Pol III.

T F The use of single stranded oligonucleotides for recombineering to effect chromosomal changes, such as gene deletion, requires oligonucleotides of 36 or more bases, base homology on both sides of the region to be deleted, and expression of the lambda Bet protein.

T F Versions of non-lytic filamentous phages fd, f1, M13, X174 and P1 that each have single-strand DNA genomes that infect E. coli cells by attaching to the F pilus are useful as DNA cloning vectors.

Ans.(top to bottom): false, true, false

Explanation / Answer

Answer =false

Justification=mismatch repair in E.coli involves a process whereby a base mismatch created by replication error is recognized by MutS & binds to mismatched base pairs.Then MutL recruited the complex.

Dam proteins recognise GATC sequence & methylates all the Adenine in this sequence.

Immediately after DNA replication all template strand has been methylated.

but daughter strands are hemimethylated

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