7. A sRNA (80 b in length) is not fully complementary to a mRNA. Can the sRNA af

Question

7. A sRNA (80 b in length) is not fully complementary to a mRNA. Can the sRNA affect the stability or the rate of translation of the mRNA.
A. No, the sRNA and and target RNA must be 100% commentary.
B. No, the Hfq protein may not help the sRNA because the RNA is loo long
C. Yes, the Hfq protein would process the long sRNA so that it can be used by the cell
D. Yes, the Hfq protein would facilitate the sRNA-target RNA hybridization and the biological outcomes

8. A mRNA has a 5’ leader sequence that folds and cover the RBS. The sRNA (named HB26A) if present in the cell hybridized with with the leader of the mRNA and free the RBS. What will be consequence.
A. The mRNA will be destroyed by the nuclease.
B. The mRNA will not be translated.
C. The rate of translation of the mRNA will be increase significantly.
D. The sRNA will be destroyed by the nucleases.

Explanation / Answer

Answer 7:

D. The Hfq protein would facilitate the sRNA-target RNA hybridization and the biological outcomes. Recent scientific research shows that the small regulatory RNAs (sRNAs) use the Hfq chaperone to mediate pairing with mRNAs and regulation of mRNAs. Thus, the sRNA, facilitated by the Hfq protein, will affect the stability or the rate of translation of the mRNA.

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