21. Which of the following is crRNA? A. The full length CRIPR RNA transcribed fr

Question

21. Which of the following is crRNA?
A. The full length CRIPR RNA transcribed from the promoter of the CRISPR leader.
B. A fragment of CRISPR RNA after the cascade complex processed the CRISPR RNA.
C. A fragment of RNA of a virus that is currently infecting the bacterial cell.
D. A fragment of the CRISPAR RNA that has the spacer sequence but no repeat sequence.

22. Which of the following is incorrect about the cascade complex of E. coli.
A. It binds virus genome and prevents transcription/translation of virus genes
B. It cuts/process the CRISPR RNA and makes crRNA
C. It uses crRNA as the guide to find out virus RNA or DNA
D. It cuts virus RNA or DNA that matches crRNA
E. It contains nearly all of the cas proteins

Explanation / Answer

Ans 21: CRISPR RNAs (crRNAs) guide the effector complex to the invading nucleic acid (of virus) and the cas proteins degrade the nucleic acid. crRNA are the fragments of CRISPR RNA . So the option B i.e. a fragment of CRISPR RNA after the cascade complex processed the CRISPR RNA is correct.

Ans 22: All of the options are correct except option D. So, option D is incorrect.

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