Could you please explain these answers to me? Especially the 1st question. Why w

Question

Could you please explain these answers to me? Especially the 1st question. Why wouldn't this partial haploid survive on a lac medium if the F' DNA has all the complimentary functional genes? Thanks!

Problem 14.22 Suppose the lac operon partial diploid cap I+P+O+ Z- Y+/ Cap T P+O+ Z is grown. (cap- indicates that the CAP-CAMP binding site is not functional.) Will this partial diploid strain grow on a lactose medium? yes no Submit My Answers Give Up Correct Part B ls transcription of B-galactosidase inducible, constitutive, or noninducible? inducible constitutive noninducible Submit My Answers Give Up Correct Part C ls transcription of permease inducible, constitutive, or noninducible? inducible constitutive noninducible

Explanation / Answer

simple concept.

For lac metabolism it needs these z and y genes.

here, in this merozygote, the first genotype, it has a mutant of z gene, due to this, it is unable to produce functional product. In the second genotype the has a mutant of y gene, due to this, it is unable to produce functional product.

If you take this merozygote, as the repressor protein works as co element (which means it can acts on cis as well as trans mode) it controls both operons. In presence of lactose, as lactose binds to repressor, it turns in to inactive one, it leads to the expresson of the other genes on that operon. the first one produces functional product for y gene, and latter one produces functional product for z gene.

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