As described in the Foundation Figure, one can consider the conversion of substr
ID: 179397 • Letter: A
Question
As described in the Foundation Figure, one can consider the conversion of substrate to product as a series of interactions with an enzyme, which serves as an organic catalyst:
E+S k1?k?1 ES k2?k?2 EP k3?k?3 E+P
Leanor Michaelis, Maude Menten, G.E. Briggs and J.B.S. Haldane simplified this equation by making assumptions. Which of the following were assumptions that helped to simplify this reaction to the form that was then used for the Michaelis-Menten equation?
Part A As described in the Foundation Figure, one can consider the conversion of substrate to product as a series of interactions with an enzyme, which serves as an organic catalyst: Leanor Michaelis, Maude Menten, G.E. Briggs and J.B.S. Haldane simplified this equation by making assumptions. Which of the following were assumptions that helped to simplify this reaction to the form that was then used for the Michaelis-Menten equation? Check all that apply. O The forward rate constant k3 can be ignored, since the rate is essentially equal to zero. The back-reaction between enzyme (E) and product (P) s negligible, making k 2 and k 3 essentially equal to zero. The binding of substrate (S) to enzyme (E) reaches a rapid equilibrium, such that the concentration of the ES complex is relatively constant after a brief mixing period. The catalytic step, k2, is slow relative to the other rate constants, making k2 the rate-limiting step in catalysis. None of these was an assumption made to simplify the above equation. Submit Hints My Answers Give Up Review PartExplanation / Answer
answer is option 2 and 3.
the reverse reaction from product to substrate is not considered because the equation describes the initial rates when [p]=0
the [ES] complex is a steady state intermediate ie., the concentration of [ES] remains relatively constant because it is produced and broken down at the same time.
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