6. Transcription: Give three examples (from either prokaryotes or eukaryotes) in
ID: 179735 • Letter: 6
Question
6. Transcription:Give three examples (from either prokaryotes or eukaryotes) in which intramolecular or intermolecular RNA:RNA base pairing is ultimately involved in regulating the level of gene expression/protein expression. (Give concise examples no explanation necessary) ......... ..........
7. Replication: . (A What is the order enzymatic events in E coli involved in the synthesis of an okazaki fragment and maturation to high molecular weight DNA?
....
(B) What is the chemical difference between the groups joined by DNA polymerase vs. NA ligase?
C))) which enzyme activity of E. coli polymerase I is responsible for removal of is matched base pairs? ( 6. Transcription:
Give three examples (from either prokaryotes or eukaryotes) in which intramolecular or intermolecular RNA:RNA base pairing is ultimately involved in regulating the level of gene expression/protein expression. (Give concise examples no explanation necessary) ......... ..........
7. Replication: . (A What is the order enzymatic events in E coli involved in the synthesis of an okazaki fragment and maturation to high molecular weight DNA?
....
(B) What is the chemical difference between the groups joined by DNA polymerase vs. NA ligase?
C))) which enzyme activity of E. coli polymerase I is responsible for removal of is matched base pairs? (
Give three examples (from either prokaryotes or eukaryotes) in which intramolecular or intermolecular RNA:RNA base pairing is ultimately involved in regulating the level of gene expression/protein expression. (Give concise examples no explanation necessary) ......... ..........
7. Replication: . (A What is the order enzymatic events in E coli involved in the synthesis of an okazaki fragment and maturation to high molecular weight DNA?
....
(B) What is the chemical difference between the groups joined by DNA polymerase vs. NA ligase?
C))) which enzyme activity of E. coli polymerase I is responsible for removal of is matched base pairs? ( ......... ..........
7. Replication: . (A What is the order enzymatic events in E coli involved in the synthesis of an okazaki fragment and maturation to high molecular weight DNA?
....
(B) What is the chemical difference between the groups joined by DNA polymerase vs. NA ligase?
C))) which enzyme activity of E. coli polymerase I is responsible for removal of is matched base pairs? (
Explanation / Answer
7.
A. The short pieces of lagging-strand DNA are named Okazaki fragments.
On the lagging strand, new initiation event takes place. This is accomplished by making short pieces of RNA at the replication fork. These RNA primers are complementary to the lagging strand template. Each primer is extended from its 3 end by DNA polymerase I to form an Okazaki fragment. DNA polymerase III catalyzes synthesis of DNA in the 5 3 direction by extending each short RNA primer.
Okazaki fragments are eventually joined to produce a continuous strand of DNA. The reaction proceeds in three steps: removal of the RNA primer, synthesis of replacement DNA, and sealing of the adjacent DNA fragments.
B. DNA polymerase is an enzyme that assists in DNA replication, by adding free nucleotides to the 3’ end of a newly-forming strand.
DNA ligase is a type of ligase that can link together two DNA strands that have single-strand breaks. The alternative, a double-strand break, is fixed by a different type of DNA ligase using the complementary strand as a template but still requires DNA ligase to create the final phosphodiester bond to fully repair the DNA.
C. Nucleotide excision activity
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