The structure of DNA DNA replication Genetic information flow The genetic code D

Question

The structure of DNA DNA replication Genetic information flow The genetic code Draw and describe the structure of a bacterial gene. Define the terms Promoter, Leader sequence, coding region and terminator sequences. Why are they important? What is an operon? Draw one. Compare and contrast inducible and repressible enzymes. Use the lac operon to describe negative control of an inducible enzyme. Use the presence/absence of lactose to explain. Use the lac operon to describe negative control of a repressible enzyme. Use arg to explain. If a bacteria is supplied with lactose and glucose what does it do? How does it choose which sugar to consume? Describe how positive control works. How is glucose a "global regulator"? You should discuss glucose levels, cAMP, and CRP. Discuss RNA based regulation using: Antisense RNA Riboswitches what is a Shine Dalgano sequence

Explanation / Answer

3. A promoter is a region of DNA that initiates transcription of a particular gene.

Leader sequence: The sequence at the 5' end of an mRNA that is not translated into protein.

Terminator sequence: A sequence in DNA that signals termination of transcription to RNA polymerase.

4. An operon is a functioning unit of genomic DNA containing a cluster of genes under the control of a single promoter.

5.  The Inducible enzyme is used for the breaking-down of things in the cell. It is also a part of the Operon Model, which illustrates a way for genes to turn "on" and "off". The Inducer causes the gene to turn on.

Repressible enzyme: An enzyme whose production is generally continuous but can be halted if a particular substance is present in concentrations greater than normal.

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