Shown is a representation of the results of an electromobility shift assay (EMSA

Question

Shown is a representation of the results of an electromobility shift assay (EMSA). The plus and minus symbols indicate which of the listed components were included in each shift assay. You know from other experiments that both ZF and SWI/SNF are required for expression of your favorite gene (YFG). Based on the data shown, propose a model as to how YFG is activated in vivo. In your answer, indicate a general role of a transcription activator and a chromatin remodeler.

24. Shown is a representation of the results of an electromobility shift assay (EMSA). The plus and minus symbols indicate which of the listed components were included in each shift assay. You know from other experiments that both ZF and SWI/SNF are required for expression of your favorite gene (YFG). Based on the data shown, propose a model as to how YFG is activated in vivo. In your answer, indicate a general role of a transcription activator and a chromatin remodeler. (6 pt) Naked DNA-labeled DNA fragment Naked DNA t t t t containing regulatory element Mono nucleosome Mononucleosome same DNA fragment assembled around ZF histone octamer AZF ZF zinc finger protein SWI/SNF AZF ZF with zinc finger deleted Sample wells SWI/SNF chromatin remodeling complex End of Gel

Explanation / Answer

Transcriptional activator i.e zinc finger protein binds to naked DNA to initiate transcription while chromatin remodelling complex i.e SWI/ SNF remodell the mononucleosomes to form naked DNA to which zinc finger proteins can bind to and initiate transcription.YGF is activated in the following way:

So the model is :

SWI/SNF binds to mononucleosomes---- mononucleosomes remodell to form naked DNA----Zinc finger protein binds to naked DNA---transcription of YGF gene.

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