Question 35 (1 point) Which enzyme is required to insert DNA into a cloning vect

Question

Question 35 (1 point)

Which enzyme is required to insert DNA into a cloning vector?

Question 35 options:

DNA polymerase

DNA gyrase

DNA ligase

DNA helicase

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Question 36 (1 point)

A circular double stranded plasmid pABC123 contains four EcoRI restriction sites. How many double stranded DNA fragments will result from cutting this plasmid with EcoRI?

Question 36 options:

1

4

5

8

10

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Question 37 (1 point)

In gel electrophoresis, which of the following DNA fragments would travel the farthest distance from the sample well?

Question 37 options:

ATCCCGAT

ATCCCG

ATCC

AT

ATCCCGATTGCACGTT

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DNA polymerase

DNA gyrase

DNA ligase

DNA helicase

Explanation / Answer

1. The answer is: DNA ligase.

DNA ligase is required to insert DNA into a cloning vector.

2. The answer is: 4.

A circular double stranded plasmid pABC123 contains four EcoRI restriction sites. Cutting this plasmid with EcoRI will result from 4 double stranded DNA fragments.

3. The answer is: AT

In gel electrophoresis, the DNA fragments "AT" would travel the farthest distance from the sample well as this has the smalles size.

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