3H thymidine is incorporated into DNA during synthesis. A culture of rapidly gro
ID: 181439 • Letter: 3
Question
3H thymidine is incorporated into DNA during synthesis. A culture of rapidly growing asynchronous cells is given 3H thymidine. The cells are then allowed to grow for 1 hour. At the end of the hour, a sample of the culture is fixed and examined by autoradiography. For the purposes of this experiment, consider that the length of G1 is 15 hours, S is 2 hours, G2 is 3 hours, and M is a half hour. Which result would you predict?
a. All of the cells that are in mitosis, but no others, have incorporated the labeled thymidine.
b. All of the cells, both mitotic and nonmitotic , have incorporated the labeled thymidine.
c . None of the mitotic cells, but some of the non-mitotic cells, have incorporated the labeled thymidine.
d. All of the non mitotic cells have incorporated the labeled thymidine
e. None of the non-mitotic cells have incorporated the labeled thymidine.
Explanation / Answer
The answer is b. All of the cells, both mitotic and nonmitotic , have incorporated the labeled thymidine. Becasue S phase is common for the all divisions.
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