Use the following word bank to fill in the blanks in the passage below regarding

Question

Use the following word bank to fill in the blanks in the passage below regarding prokaryotic translation (not all answers will be used): Translation in prokaryotes is initiated when _ binds to the 30 S subunit of a ribosome which recognizes the _ sequence of an mRNA. Next, an initiator f Met-tRNA, that contains a _ group, gets bound by _ and GTP and binds to the first AUG in the mRNA. After all of the initiation factors dissociate and GTP is hydrolyzed, the _ associates with the small ribosomal subunit. Next, a tRNA, bound by the elongation factor _ and GTP, binds to the _ site of the large ribosomal subunit, leading to the hydrolysis of GTP and dissociation of the elongation factor. A _ bond is formed between the amino acids on the tRNAs and the ribosome shifts forward 3 nucleotides with help from EF-G. This process continues until the ribosome gets to a stop codon, at which point _ binds to the stop codon and _ binds to the large ribosomal subunit, which leads to the disassembly of the complex.

Explanation / Answer

Translation in prokaryotes is initiated when 50S subunit binds to the 30S subunit of a ribosome which recognizes the methyl sequence of mRNA. Next, an initiator fMet-tRNA, that contains a formyl group, gets bound by IF-2, IF-3 and GTP and bind to the first AUG in the mRNA. After all the initiation factors dissociate and GTP is hydrolyzed, the Shine-Dalgarno associates with small ribosomal subunit. Next, a tRNA bound by the elongation factor EF-Tu and GTP, binds to the P site of the large ribosomal subunit leading to hydrolysis of GTP and disassociation of elongation factor. A peptide bond is formed between the amino acids on the tRNA and the ribosomes shifts forward three nucleotides with help from EF-G. This process continues until the ribosome gets a stop codon, at which RF-1/RF-2 binds to the stop codon and RF-3 binds to the large ribosomal subunit, which leads to disassembly of the complex.

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