First & Last Name: You find a human homologue of protein X. You amplify the gene
ID: 182245 • Letter: F
Question
First & Last Name: You find a human homologue of protein X. You amplify the gene for this protein (gene X), c into a cukaryotic expression vector and transfect it into cancer cells. As a control, you use a vector 4. which does not contain gene X (empty vector). You lyse cells that were transfected with gene X plasmid, as well as the control (empty vector) at the indicated hours (shown below above each figure) after transfection and perform a Western blotting experiment using anti-cyclin antibodies. Which of the two figures do you expect to see for cells transfected with the gene X plasmid and the control plasmid (top or bottom) ? Please explain your logic briefly. Hours after transfection 26 36 46 56 66 76 86 96 106 116 126 136 146 2Explanation / Answer
Answer : graph 2
Explanation: Cycins are those cell division regulatory proteins which are gradually generated and destructed in the cells with respect to the pattern of cell division. It must be carefully recalled here that the time pattern of cellular division is fixed and highly regulated for each cell type, including cancer cells. Thus, as the cell cycle will progress, the extent of cyclin expression should also follow a pattern. The graph 2 clearly suggests that the maximum concentration of cyclin is observed after 46, 86 and 126 hours i.e. every 40 hours which indeed follows a pattern of cell division. This pattern is not observed in first graph. Hence, the synchronization of cell with cyclin concentration is observed in graph 2, hence it is the correct choice.
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