Refer to the information in the table below for the following questions Molecula

Question

Refer to the information in the table below for the following questions Molecular Weight (Daltons) Number of Residues pl 9.5 Chymotrypsin (bovine 25,200 pancreas) Cytochrome c (human) 12,400 Hemoglobin (human) 64,500 Lysozyme (chicken egg 14,300 white) Myoglobin (equine heart) 16,700 Serum albumin (human) 66,00 Titin (human) Transferrin A-antitrypsin Pepsin 241 104 574 129 10.7 6.8 11.0 2,993,000 71,800 44,300 42,100 153 609 26,926 641 418 390 7.0 4.9 6.3 6.9 5.2 If you were to use size exclusion chromatography to separate the ten proteins in the table above, in what order would the proteins appear during elution from the column?

Explanation / Answer

Please find the answers below:

Answer 1: According to the information, size exclusion chromatography is used to separate a mixture of proteins. Here, it must be recalled that proteins of light molecular weights are eluted first followed by proteins of large molecular weights later. Thus the order of elution can be found as below:

Answer 2: Choice 1 is correct.

Explanation: Size exclusion chromatography is based upon the fact that difference in molecular weights is used to elute tge target proteins from a mixture. Hence, proteins of comparable molecular weights become difficult to elute. Here, molecular weights of A-antitrypsin and pepsin are too close to elute them effectively from a size exclusion column.

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