8. You are designing a green fluorescent protein (GFP) variant that displays on

Question

8. You are designing a green fluorescent protein (GFP) variant that displays on the surface of a liposome. Suggest 3 possible modifications that would allow it to be attached to the surface of the liposome. 9. You are designing different liposomes for drug delivery through the blood stream and for skin application as a topical lotion. Describe your selection of lipids for each application that would ensure the liposomes remain in a semi-fluid state between liquid-ordered and liquid-disordered states 10. You scratched your finger on a rusty nail and have been infected by Clostridium tetani. If you fail to seek treatment, what will happen? Which proteins will be targeted?

Explanation / Answer

ANSWER-8 : 3 POSSIBLE MODIFCATIONS NEEDED:-

(a) need to add some mutations that can enhance folding, brightness, and also reduce environmental sensitivity

(b) GFPs needed to be added along with protein's sequence and also it can be added within loops or flexible domains of the proteins

(c) good linkers are needed to be created consisting of glycines interspersed with serines or threonines that prevents any interference in protein binding

ANSWER-9: criterias for the selection of lipids:-

a) must be physiologically active and lipophillic

b) should have a good release rate so that maximum drug is contained inside the liposomes

c) lipids should be phospholipids with unsaturated acyl chain 0 moieties

ANSWER-10 : Infections with C. tetani produces exotoxins such as tetanolysin and tetanospasmin.Tetanospasmin is a neurotoxin and may lead to tetanus.

It targets the synaptobrevin-2 (also known as VAMP2) which is a SNARE protein by causing cleaving the protein.

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