B. You have purchased a protease that can specifically cleave the Gln-Gly peptid

Question

B. You have purchased a protease that can specifically cleave the Gln-Gly peptide bond of the GluAsp-Asp-Tyr –Asp-Gln-Gly sequence present in any protein (and it cleaves no other sequence found in proteins). Describe how you can use this protease (in combination with other purification method/s) to obtain pure protein A starting from the purified mixture of proteins A, B, and C you obtained in sub-question 2A. Assume that you will only need a very small amount of protease to cleave a large amount of its target protein, so the remaining protease is an insignificant impurity. (1 point)

Explanation / Answer

Here the subquestion 2A is not mentioned

In this experiment we use proteiase and allows proteins break down into peptides.

These protein mixture is alllowed to run on gel electrophoresis.

We can use different kind of gel electrophoresis like SDS gel, Native gel, 2D gel electrophoresis and many others.

By use of nontreated and treated protein we can compare it and then seperate.

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