1. Later this week the concentration of your DNA extraction will be quantified u
ID: 185250 • Letter: 1
Question
1. Later this week the concentration of your DNA extraction will be quantified using a UV spectrophotometer by measuring its absorbance in units of optical density (OD). DNA absorbs light most strongly at the ultraviolet wavelength of 260 nm. The absorbance of double stranded DNA (dsDNA) at 260 nm (A260) is used to estimate concentration, with an OD of 1.0 equal to a dsDNA concentration of 50 g/ml. If a sample of double-stranded DNA was diluted 50X, and gave an A260 reading of 0.70 OD on a spectrophotometer, what is the concentration of dsDNA in the original sample? Use the following calculation to obtain your answer, and show your work: dsDNA concentration = 50 g/ml x OD260 x dilution factor
(part 2) If you eluted the DNA sample used in question 1 in 200 l of buffer what is the total amount of DNA you extracted from your sample? Show your work, and include units in your final answer.
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