NAME DATE BIOL 200 LABORATORY MATH 1. Dr. Haffrey told his student, Amanette, to
ID: 186202 • Letter: N
Question
NAME DATE BIOL 200 LABORATORY MATH 1. Dr. Haffrey told his student, Amanette, to make 300 mL of a 2M concentration of NaC Amanette did not want to calculate how much salt was needed by using the molecular weight of the substance. Instead, she searched the laboratory for a concentrated solution and found Zachary's 4M stock solution of NaCl. a) How much of the 4M stock solution did Amanette use? Show your work. (5 points) b) How much of the diluent did Amanette use? (5 points) c) What was the diluent that Amanette used? (2 points) 2. Amanette and Jason were arguing which solution had more potassium. Amanette argued that 20 mL of a 3096 solution had more than 10 ml of 50% solution. Jason disagreed. a) Who is correct? (2 points) b) Justify your answer in the space provided below. (5 points) Jason had to calculate the dilution factor in order to plate serial dilutions his concentrated cells as the correctly plating volume. He wanted his experiment to be consistent with his previous results. He saw that his starting concentration was 8 million cells/mL. The volume of the he use before dilution was 1 mL and the volume that he used after the dilution was 20 a) What is the dilution factor he used? Show your work. (5 points) 3.Explanation / Answer
Answer 1] a] given things are
C1= Concentration of stock solution
V1=volume of stock solution needed to make new solution
C2 =final conc.of new solution
V2=final volume of new solution
C1V1 =C2 V2
V1 X( 4M) = (300ml) (2M)
V1 = 300 X 2 / 4
=150 ml
150 ml of stock solution of 4M should amanete used.
b] He used 150 ml of stock solution & 150 ml of Diluent is used to make a final volume of 300 ml solution.
c]The diluent is the distilled water that amanette used.
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