Which of the following statements is NOT a correct description of the DNA meltin

Question

Which of the following statements is NOT a correct description of the DNA melting temperature for a system in which we have equal molar concentrations of two distinguishable strands?

Select one:

a. It's the temperature at which the molar concentrations of the strands (S and S ) and the duplex (D) are related by the following equation: [S + [S = 2[D].

b. It’s the temperature at which half the DNA strands have associated with one another to form duplex DNA.

c. It's the temperature at which the absorption at 260 nm is midway between that of single-stranded DNA and that of double-stranded DNA.

d. It’s the temperature at which the mass of single stranded DNA equals the mass of double stranded DNA.

e. It’s the temperature at which single stranded and double stranded DNA have the same standard free energy.

f. It's the temperature at which the equilibrium constant for duplex formation is the inverse of the total strand concentration times 4.

g. None of the above (i.e., all the above are correct descriptions of the DNA melting temperature for a system in which we have equal molar concentrations of two distinguishable strands).

Explanation / Answer

Melting temperature is considered as the temperature at which half of the DNA strands are denatured.

All the above options are correct except option c. It is so because nucleic acid starts UV absorption at 260nm. The absorbance of the DNA at 260nm increases as the melting starts.

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