Proteins are chains of amino acids. There are twenty different types of naturall
ID: 186364 • Letter: P
Question
Proteins are chains of amino acids. There are twenty different types of naturally occurring amino acids, and all organisms must have all twenty of them in order to survive. In this project we will be discussing the amino acid leucine. What are the two possible methods that a cell may employ to be certain that it has each of the 20 amino acids?
RNA is partly made of the base uracil. What are the two possible methods that a cell may employ to be certain that it has uracil?
Suppose that you have a particular S. cerevisiae mutant strain that cannot make the amino acid leucine. Will the strain grow on a YPD plate? What about a SD plate? How could you modify the SD plate to allow this strain to grow
Explanation / Answer
Ans- two possible method for a cell to be certain that it will have all the 20 amino acid is introduction of a new DNA sequence through 1) electroporation or 2) gene gun method.
The same methods can be used for the RNA to certain to have uracil.
The species of mutant S. Cerevisiae will grow easily on the YPD plate Because it contain all the amino acids.
They can't grow on SD media as it doesn't have amino acids source, it should be modified by introduction of essential amino acids for growing such mutant strain.
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