microbiology 233 microbiology 233 microbiology 233 1. What effects may heat fixa

Question

microbiology 233 microbiology 233 microbiology 233 1. What effects may heat fixation have on the characteristics of a microbe? 2. What information can be obtained from simple staining technique? 3. What electrical charge is on bacterial cell wall? 4. What are acid or basic dyes? Which type of dye did you use in this experiment and what electrical charge did the dye carried? 5. Why air drying the slides is important before heat drying? 6. Why the inoculating loop must be heated to red hot before placing back on the lab table.

Explanation / Answer

2) simple staining can tell us whether the bacteria is a gram positive bacteria or gram negative.. It can tell us the nature and composition of the cell membranes of the particular microbes...

3) the charge can be positive also.. It can be negative also.. It depends on the composition of the membrane on the microbes..

6) it basically kills all the microbes which can be attached on the loop while doing the experiment so that the next person who can use ensure that there is no contamination from the previous experiment...

1) heat fixation help in the penetration of dye into the microbes membrane so that it can easily get stain especially in the case of malachite green spore staining which help to penetration of the malachite green dye into the hard cover of spore..

4) acidic dye which has negative charge over it and can bind to the positive charge membranes microbes.. Basic dye which has positive charge and can bind or stain to the negative membranes microbes...

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