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80% 1 t.ed ork 2.1 (Chapter 3) 01/25/18 88% O See Hint Imagine that you are an e

ID: 186547 • Letter: 8

Question

80% 1 t.ed ork 2.1 (Chapter 3) 01/25/18 88% O See Hint Imagine that you are an early microbiologist attempting to reproduce Louis Pasteur's famous experiments which helped disprove spontaneous generation. You first prepare a hay infusion (a type of nutrient broth), then boil it for 20 minutes in a swan-necked flask of the same design that Pasteur used. Upon cooling and incubation at room temperature, you notice that your broth has become cloudy (turbid) only a few days later. What did your broth contain that Pasteur's likely did not? Choose one: O A. hyperthermophilic archaea B. sufficient oxygen O C. sufficient nutrients D. akinetes E. endospores O F. myxospores 1st attempt VIEW SOLUTION

Explanation / Answer

Choice B is correct.

Reason: The primitive earth had a highly reducing environment suggesting that there was severe deficiency of oxygen. This is why only those organisms and primitive cells could survive on the primitive earth which were either anoxygenic in nature or did not require oxygen as the vital gas. This phenomenal fact was replicated experimentally using a swan-necked flask to restrict the oxygen content in the broth. However, the set up of the present experiment is rich in oxygen due to un-interrupted supply and hence bears different results from expected.

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