After making these serial dilutions, we will plate the dilutions onto LB plates
ID: 186718 • Letter: A
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After making these serial dilutions, we will plate the dilutions onto LB plates in order to allow the bacteria to grow. We assume each bacterial colony originates from only 1 bacterial cell, so we assume 1 colony is equal to 1 cell. To determine cell density (cells/ml) of the original culture take the # of colonies on a plate divided by the volume plated for that dilution. Then, multiply the cell density of that dilution by the overall dilution by the overall dilution factor to get the cell density of the original culture.
1. Imagine that you count 37 colonies on the last plate (i.e. the one on the far right). Theoretically, how many colonies would you expect to see on the second to last plate?
2. What would the cell density of the original bacterial culture be if there were 37 colonies on that last plate?
3. How many total cells are in the initial bacterial culture if there was 50 ml in the original bacterial culture?
Il ut dTuview from MICRB 202/203) If a solution is too concentrated, then dilutions can be mad particles found in a particular volume. However, manageable, sometimes we need to do what is called serial din is used to make another dilution that is then use in order to count the number of colonies on would be too many colonies to accurately count by eye en ditutions can be made to decrease the number of ar volume. However, in order to keep the dilution volumes s we need to do what is called "serial dilutions" where one dilution ne another dilution that is then used to make another dilution. We will do this mber of colonies on a plate. If we did not perform dilutions, there! In order to calculate the "overall dilution" for an calculate the "overall dilution" for any sample that has been diluted multiple you can simply multiply the dilution factors for each single dilution. If a murallon value is known for a diluted sample, then the concentration of the original sample can be determined by multiplying the diluted concentration by the overall dilution factor for that dilution. Below is a diagram that outlines the serial dilutions that you will be performing for your bacterial culture. 7. Calculate the "single" dilution factors and “overall" dilution factors for each tube. 0.1 ml 0.1 ml 1.0 ml 1.0 mlExplanation / Answer
Approx 28-32 colonies will appear in the plate 2.
2. 37*10 power 9
3.Approx we can say that 350- 400 cells are present in one ml of that original culture.
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