1. Soon after the discovery of restriction enzymes, it was found that some restr
ID: 18692 • Letter: 1
Question
1. Soon after the discovery of restriction enzymes, it was found that some restrictionenzymes produced DNA fragments which averaged several hundred base pairs in
length, other produced fragments that averaged several thousand base pairs in
length, and still others produced fragments that we extremely large.
a. What is the main factor determining the average size of the DNA fragments
produced by any restriction enzyme? That is, what determines how
frequently a restriction enzyme cuts DNA?
b. What are some reasons that a molecular biologist might want to get DNA
fragments of a particular average size?
c. What would you predict the average size of fragments produce by the
following restriction enzymes would be?
Sau-IIIa
Eco-R1
Not-1
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