8. Support the statement: \"DNA replication is semi conservative. 9. Discuss the

Question

8. Support the statement: "DNA replication is semi conservative. 9. Discuss the methylation-dependent mismatch repair system. What enzyme plays an essential role in this repair system. 10. Discuss the different effects of base pair substitutions. 11. Discuss the advantages of using prokaryotes in genetic studies. 12. Describe in detail at least 3 ways in which microorganisms have a direct and substantial impact on your life (other than as causative agents of disease). Include a discussion of the role(s) of the microorganisms in these processes. 13. Discuss the role of the proteins involved in the formation of the clamp. 14. What is a plasmid? Describe the structural characteristics of plasmids and explain the roles they play in bacterial cells. 15. Differentiate between an exonuclease and an 16. Differentiate between these 2 types of mutations- inversions and translocation 17. Distinguish between point mutations and frameshift mutations. 18. A DNA molecule consists of 2000 base pairs. 40% of the base pairs are A-T How many bases in the DNA molecule are guanine? 19. From the DNA base sequence of ATACCGTAC, write an example of a deletion mutation and an inversion mutation. 20. Explain one strategy for an experiment proving that the replication of DNA is semi-conservative (see text book). 21. Discuss the structure of DNA. What is DNA composed of? What is a deox Draw the structure. Discuss base-pairing rules. e?

Explanation / Answer

8.Replication is process of the formation of carbkn copy of DNA by the help of enzyme DNA polymerase. By the process of replication gentic material is doubled and one set of chromosomea is passed to offspring. DNA replication is semiconservative in nature and newly formed DNA molecule contains one strand synthesised newly and one from the parents.hence called semi conservative.The semi conservative nature was explained by Measalson and Stahl by an experiment. They used radioactive nitrogen to enter into DNA for severas genratiogen and then after one cell division they examined and given the result that DNA replication is semiconservative in nature.

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