Assess the usefulness and limitations of information obtained via different type

Question

Assess the usefulness and limitations of information obtained via different types of microscopy Describe different strategies to isolate cellular organelles and macromolecules (DNA/protein) Analyze the applications of recombinant DNA technology Understand how transgenic and knockout mice can be used for the study of cell biology Describe the process and applications of immunofluorescence Compare direct (primary) and indirect (secondary) immunofluorescence Explain how the field of bioinformatics can be used for studying whole genomes /proteomes Describe how cell culture and model organisms can be used to study cellular processes Explain how cells can be made to grow in culture indefinitely

Explanation / Answer

The most economically accesible microscopes are the light microscopes (they use visible light to form images): Compound microscope and Stereoscope.

The compound microscope has high magnification, you can observe individual cells with them, but the image is two dimensional and they have low resolution.

The stereoscope is also knows as dissection microscope. The image is three dimensional. It's very useful for dissections, it allows you to get a better look at a larger specimen, but it has low magnification, you can't view cells.

There are also electron microscopes that use electrons for generating images. There are two types: Scanning electrone microscope (SEM) and Transmission electrone microscope (TEM). Both have high magnification and resolution, but their limitation is that they are very expensive. The scanning electrone microscope generates 3-D images, while the transmission electrone microscope is 2-D.

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