lii then incorporate the non natural amino acid during tural amino acids provide
ID: 187756 • Letter: L
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lii then incorporate the non natural amino acid during tural amino acids provide chemical groups that can cross-link in Non na different parts of the protein with UV light or chemical modifications. In addition, some non native amin function until exposure to light In addition. non n the protein by spectroscopy o acids are "photo caped and do not atural amino acids are used to "visualive In summary protein engineering is essential to many biotechnology applications t creates more stable enzymes for inslustrial use. enzymes with new substrate or cofactor speciicity, neel enzymes with directed evolution, combinatorial libaries or the use of non natural amino acids In addition, protein engineering creates new biomaterials in the medical field, such as elastin like peptides and binding proteins that target a dnug or reagent to a specific tissue ox ongan End-of-Chapter Questions 1. What is protein engineering? a. alteration of a protein through genetic engineering to improve the protein's properties b. modification of the amino acid residues after a protein has been translated c. engineering a protein to be expressed in an artificial expression system d. production of large quantities of proteins for use in industrial settings e. none of the above 2. Which alteration to the protein sequence provides a major increase in protein stability? a. glycosylation of amino acid residues b. acetylation of amino acid residues c. oxidation of the protein d. introduction of cysteine residues for disulfide bond formation e. reduction of the protein 3. Which of the following concepts increases protein stability? a. decreasing the number of possible unfolded conformations b. decreasing the number of hydrophobic interactions c. increasing the number of hydrophilic interactions d. decreasing the number of disulfide bonds e. none of the above 4. What is a benefit to changing the active site or cofactor binding site of an enzyme? a. to prevent a reaction from taking place b. to produce the enzyme's product more cost-effectively c. to make the enzyme more stable d. to ensure that only the native substrate or cofactor are able to bind to the enzyme none of the above e. 5. Why might it be sensible to simplify an industrially relevant enzyme? a. to decrease the possibility of misfolding in the protein b. to increase the amount of protein being made c. to increase efficiency of the enzyme d. to decrease the amount of amino acid supplements to the growth medium for the growing cells none of the above e. Continued)Explanation / Answer
1. What is protein engineering....
a. Alteration of protein through genetic engineering to improve protein's properties.
2. Which alternation in protein sequence provide major increase in protein stability..
a. Glycosylation of amino acid residue play a critical role in determining protein structure function and stability
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