Academic Integrity: tutoring, explanations, and feedback — we don’t complete graded work or submit on a student’s behalf.

P52 tor Canvas (2) Word Nth Raba HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES M

ID: 187907 • Letter: P

Question

P52 tor Canvas (2) Word Nth Raba HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES MAILINGS REVIEWVIEW Cut Replace Select . Paste Format Painter B , u, kex, x A,. . :EE , ,n· 11Normal 1N0 Spac Heading 1 Heading 2 Title Subtitle Subtle Em Emp hasis Intense E Strong Ouote sly es Bonus question What is the size of the processed mature mRNA of this gene if the paly-A Question a) (4 pts). tail is 83 A long? 5UTR Intron Exon Intron Exon Intron Eon3UTR 365bp 57bp 85bp 133bp 77bp 42bp 72bp Exon 27p 123bp polx site 8:12 PM 20, 1/29/2010 Type here to search

Explanation / Answer

Mature mRNA contains 5'UTR + CDS + 3'UTR + Poly A tail
Introns are excised from the hnRNA during splicing in the nucleus.

Size of 5' UTR = 27 ntd
Size of 3' UTR = 72 ntd
Size of CDS = 123 + 57 + 133 + 42 = 355 ntd
Size of poly A tail = 83 ntd

Mature transcript size = 27 + 72 + 355 + 83
= 537 ntd

Mature transcripts also contain a 5'-guanosine cap. If you consider that also, the size = 538 ntd

(You should not use the word 'basepair' for mRNA as it is single stranded)