please answer all the questions for micro lab: Exercise 1-1 1, What areas were m
ID: 188002 • Letter: P
Question
please answer all the questions for micro lab:
Exercise 1-1
1, What areas were most thoroughly cleaned by your washing technique?
2. What areas were most difficult for you to clean with your washing technique ?
3.In general were your two hands cleaned an equal amount, or was one cleaned more than the other? What could account for any differences?
4.How do your answer to question 1,2, and 3 compare to your lab partner’s answers? Why might they differ?
5.Why were you instructed to have your lab partner turn the water on and off operate the UV lamp rather than you doing these actions yourself?
6.Why might it be advisable to modify the procedure and use the UV light to check your hands prior to application of lotion and the paper towels prior to drying ?
Exercise 1-2
1.Which agent seemed to be most effective at removing microbes from the fingertips?
2.Which agent seemed to be least effective at removing microbes from the fingertips?
3.What other variables besides the cleansing agent might be responsible for any differences noted between fingers or students?
4.Did you or any other students notice some microbes persisting more than others after washing? What factors might account for this?
Common aseptic transfers and inoculation methods
1.did you get growth on/in the strile nb and na slant tubes you practiced with? If not, congratulations! If so, where did you see it and what might have been its sources?
2.Considering the cultures used to inoculate each medium in this exercise, how many different microbial types should you expect to see on/in each medium? Explain your answer
Streak plate methods of isolation
1.Did you achieve isolation using the quadrant streak? If so in which streak (1,2,3,4) did it occur? If you did not achieve isolation , what might you do differently next time to improve your results?
Colony morphology
1.A description of colony morphology provides important information about an organism. What other colony features should you include ?
2.Three critical aspects of a description of bacterial growth are colony size, color and shape. At least three other important factors- not descriptions of the organism itself-typically are included when describing bacterial growth. What are they and why are they important..
Explanation / Answer
1-1
1. fingers, palms, wrist.
2. cloths.
3. two hands are equally clean.
4. same, because we both use same washing technique.
5. after washing my hand, if i touch the switch to on UV, then sterility will not be maintained. so i need my lab partner to do that.
1-2
1.most effective 95% ethanol
2.least effective soap
3.presence pf long nails, rings, bracelets, watches can hamper the cleaning up.
4. fungal, bacterial spores remain even after washing, beacuse they are resistant structures.
No, i have never seen bacterial growth on sterile nb, na tubes. but if there is growth, then may be autoclaving was not right. or handling of sterile media was poor.
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