QUESTION 8 Given that you set up your PCR reactions correctly, would you expect

Question

QUESTION 8

Given that you set up your PCR reactions correctly, would you expect to see a band for GMO negative control (plant) template DNA amplified with PSII primers and with GM primers? Choose the single, best answer:

PSII no, GM yes

PSII no, GM no

PSII yes, GM yes

PSII yes, GM no

QUESTION 9

Given that you set up your PCR reactions correctly, would you expect to see a band for GMO test food (plant) template DNA amplified with PSII primers and with GM primers? Choose the single, best answer:

PSII no, GM yes

PSII yes, GM no

PSII no, GM no

PSII yes, GM yes

PSII no, GM yes

PSII no, GM no

PSII yes, GM yes

PSII yes, GM no

Explanation / Answer

8.
Answer:
Option D is correct.

Explanation:
Here,
Template = GMO negative control plant, so GM primers can not anneal to the template.
PSII primers can anneal to non-GM plant DNA.

9.
Answer:
Option A is correct.

Explanation:
Here,
Template = GMO positive plant, so GM primers can anneal to the template.
PSII primers can not anneal to the GM plant DNA.

(These questions are answered based on the assumption that GM gene disrupts PSII in the genome).

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