Question 17: Ampholyte Buffer maintains a stable pH gradient and proteins migrat

Question

Question 17: Ampholyte Buffer maintains a stable pH gradient and proteins migrate based on charge and stop at the pH that equals protein’s: pKa-1. pKa-2. pKa-3 pI. N-terminal pKa.

15: What was the best method for this purification?

Subcellular fractionation.   b. Ammonium sulfate precipitation.   

13: Which procedure was the best in increasing purity:

Size Exclusion Chromatography.   b. Salt precipitate.   c. Ion Exchange.

d. Affinity Chromatography.    e. Acid Precipitate.

Gel filtration chromatography.    d. Ion exchange chromatography.

e. Affinity chromatography

12: The size exclusion chromatography step:

destroyed the protein.   b. increased the specific activity.

c. increased yield.   d. was a total waste of time here, but could be used in a revised purification as an earlier procedure step because it was safe

Explanation / Answer

15.Correct answer is:b.ammonium sulfate precipitation

Reason:Above method would be best to purify the protein.

13.The correct answer would be:a.size exclusion chromatography

Reason:This chromatography shows good sensitivity with no sample loss as the solutes do not interact with the stationary phase.

12.Correct answer would be: c.increased yield

Reason:This chromatography increases yield as there is no sample loss.

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