A 0.00000001 dilution is performed on a culture of bacteria in order to perform

Question

A 0.00000001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL of solution is plated on solid media, and 101 colonies of bacteria grow orn the petri dish Assuming each colony came from a single bacterium, hovw many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. * Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed * Any time more than 1 mL is transfered, a concentration is being performed

Explanation / Answer

101 colonies correspond to 101× 109 bacteria (multiplied by the dilution factor)

Dividing this by the total volume of the original culture shall give the number of bacteria per ml.

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