(I think B is the answer... but not entirely sure... ) Which of the following st
ID: 188863 • Letter: #
Question
(I think B is the answer... but not entirely sure... )
Which of the following statements describes the most suitable technique to test if intermediate filaments are dynamic?
A. SDS-PAGE and Western blot analysis to see if the intermediate filament is phosphorylated
B. FRAP in cells expressing a GFP-tagged form of the intermediate filament
C. Immunofluorescence analysis with primary antibodies to the intermediate filament and appropriate fluorophore tagged secondary antibodies
D. live cell imaging of cells expressing a GFP-tagged form of the intermediate filament
E. FRAP in cells in which intermediate filaments have been detected with primary and secondary antibodies.
Explanation / Answer
Answer:
B. FRAP in cells expressing a GFP-tagged form of the intermediate filament
Explanation:
Fluorescence recovery after photobleaching (FRAP) will help to determine the kinetics of proteins. Once we tag the intermediate filaments with GFP which is a fluorescent protein, we could use FRAP method to document the dynamic nature of the intermediate filaments.
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