Yolanda is trying to insert the gene for Green Fluorescent Protein (gfp) into mi

Question

Yolanda is trying to insert the gene for Green Fluorescent Protein (gfp) into mice. After her most recent attempt, she obtains three mice. She obtains DNA from all of the mice and performs PCR to determine whether gfp is present. She expects a band at 500 bp when she runs the reactions on an agarose gel. Her results are shown below. Yolanda shows the results to her colleague Dorothy. Dorothy says the results indicate that Yolanda must not have isolated DNA correctly. Yolanda says the results indicate that gfp was not inserted into the mice. Who is correct? CHOOSE THE SINGLE BEST ANSWER. 10000 8000 6000 _ 5000 4000 _ 3000- 2000_ 1500_ 1000 500 Cannot be determined from this information Yolanda Dorothy

Explanation / Answer

The green flourescent protein is not inserted into mice. This is because, the band appears in marker DNA but not in mice DNA. Basing on this information, Yolanda is correct. There must be some thing wrong in her approach of inserting the gene and therefore, she could not find Gfp encoding gene at 500bp as she is expecting. Option 2 is correct.

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