9. Which of the following statements describes the most suitable technique to te
ID: 188882 • Letter: 9
Question
9. Which of the following statements describes the most suitable technique to test if intermediate filaments are dynamic? (1 point) O SDS-PAGE and Western blot analysis to see if the intermediate filament is phosphorylated O FRAP in cells expressing a GFP tagged form of the intermediate filament OImmunofluorescence analysis with primary antibodies to the intermediate filament and appropriate fluorophore tagged secondary antibodies live cell imaging of cells expressing a GFP tagged form of the intermediate filament FRAP in cells in which intermediate filaments have been detected with primary and secondary antibodies.Explanation / Answer
ANSWER 9: FRAP analysis with a fluoroscently labelled stain or GFP can detect the speed and movement of intemediate filaments which are highly dynamic cytoplasmic structures.
ANSWER 4: GDI prevents cell migration as higher GDI decreases GTPases activity by preventing a chemoattractant to bind with the receptor molecule.
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