A scientist wanted to determine the protein concentration of an unknown sample.

Question

A scientist wanted to determine the protein concentration of an unknown sample. He added varying amounts of a 1 mg/ml solution of borne serum albumin and water to a series of tubes as shown in the following table. He also added 100 mu l portions of several dilutions of an unknown sample to other tubes 2.0 ml of the Bradford Reagent were added to each tube and the color was allowed to develop The absorbance at 595 nm was then determined The results are shown in the following table. Tube sample water Bradford Reagent A595 0 mu 1 BSA 100 mu l 2 0 ml 000 1 99 2.0 0.076 5 95 2.0 0378 10 90 20 0810 20 80 20 1610 25 75 2.0 1920 100 mu 1 unknown, undiluted 0 2.0 3600 100 mu l. unknown. 1/5 dilution 0 2.0 1600 100 mu l, unknown 1/10 dilution 0 2.0 0.750 a Calculate the amount of BSA in each of the first six tubes.

Explanation / Answer

Recall that the BSA is 1 mg/ml or 1 ug/uL Hence you can simply multiple the volume in uL by 1ug/uL to obtain the amount of BSA. Amount of BSA in each tube: Tube 1: 0 ug Tube 2: 1 ug Tube 3: 5 ug Tube 4: 10 ug Tube 5: 20 ug Tube 6: 25 ug

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