1. What is the purpose of using aseptic technique when handling microorganisms?
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Question
1. What is the purpose of using aseptic technique when handling microorganisms? 2. What is the name of the instrument that allows the aseptic transfer of microorganism in the microbial lab? 3. Why is it important for you to flame this instrument 4. Why is it important to allow this instrument to cool 5. Why is it important for you to keep your media 6. Agar plates should always be labeled on the top or between inoculations? completely before picking up inoculum? closed except only while inoculating fresh media? the bottom of the plate?Explanation / Answer
1. Aseptic technique is a fundamental and important lab skill in the field of microbiology. This is used for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Proper use of this technique prevents contamination of cultures from foreign bacteria inherent in the environment.
2. Sterile instruments are used for aseptic transfer of microorganisms like a sterile loop.
3. The sterile loop is sterilized with flame or another heat source until it becomes hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination.
4. After flame sterilisation, the loop must be cooled so that the next cells the loop touches are not killed by the hot metal.
5. By keeping media closed we avoid contamination by other microbes.
6. Agar plates are labelled on the bottom rather than on lid to preserve area to observe the plate after it has incubated.
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