biology in focus Questions 27-31 In a classic experiment from the 1970s in rabbi

Question

biology in focus Questions 27-31 In a classic experiment from the 1970s in rabbit a-hemoglobin vestigating gene expression, a solution containing equal amounts of mRNA and B-hemoglobin mRNA, which encode subunits of a protein found in red blood experiment. Tadpoles that developed from the injected eggs were dissected into two fragments, one containing into newly fertilized frog eggs. The injected mRNA was not degraded during the course of the ly the notochord, muscle tissue, and nerve tisue and the other containing predominantly the other nt tissue types. Equal amounts of total protein were analyzed after separation by electrophoresis to identify the relative amounts of the different proteins present in each sample. The thickness of the bands indicates the relative amounts of rabbit a-hemoglobin, rabbit hemoglobin, and frog tubulin (a cytoskeletal protein that is expressed at relatively onstant levels in all tissues) present in each tadpole sample. The experimental protocol and results are summarized in the figure below Reared to Tadpoles Injected Hemoglobin into Eggs Rabbit mRNA Tadpoles Dissected Notochord, Muscle, and Nerve Tissues Other Tissues Protein Isolation and Analysis Notochord, Muscle, and Nerve Tissues Other Tissues Frog Tubulin Rabbit hemoglobin! Rabbit-hemoglobin |- GO ON TO THE NEXT PAGE -18-

Explanation / Answer

The observation that the rabbit mRNA was successfully translated in the frog tissue supports the following conclusion:

(D) Rabbits and frogs share a common genetic code for expressing heritable information

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