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OLIVE QUESTIONS 1/ If you had a 0.1 M stock solution, can you maks 1 M working s

ID: 189351 • Letter: O

Question

OLIVE QUESTIONS 1/ If you had a 0.1 M stock solution, can you maks 1 M working so 2/ Fill out in the table below: Stock Final n-fold Dilution Final Volume V Volumes of Concentration concentration of Working Stock and SolutionWater to Pipet 1 M 0.1 M 10 mL (+90 ml water) 10 100 mL 2.2 M 9.1 mL + 40.9 mL water) 0.4 M 5.5 50 mL 0.5 M 3 M 0.1 M 0.2 M 3 mM 1.5 M 100 mM 60 mM 100 mL 100 mL 300 mL 200 mL Extra credits: the actual concentration of Crystal Violet is 7.4 mM (7.4 mmoles/L). Given that the Molecular Weight is 407.979 g/mole, calculate the actual amount or mass of Crystal Violet in micrograms and nanograms. Show your work here:

Explanation / Answer

Answer: If we have a stock solution of 0.1M, we can’t make 1M working solution from it. It is due to the fact that working solutions are made from stock solution by diluting them and applying formula like N1V1=N2V2 or C1V1=C2V2. In the present case, stock solution is already 10 times less (0.1M) concentrated (diluted) compared to working solution (1 M, more concentrated).