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In microscopic examination of eukaryotic microbes ( flgae – yeast) 1. What featu

ID: 189579 • Letter: I

Question

In microscopic examination of eukaryotic microbes ( flgae – yeast)

1. What features did the cells you observed have in common ? how were they different?

Simple stains

1. What are some consequences of leaving a stain on a bacterial smear too long ( over staining) ?

2. What are some consequences of not leaving a staining on a smear long enough ( under-staining) ?

3. Consider a coccus and a rod of equal volume.

a. Which is more likely to survive in a dry environment? explain you answer

b. Which would be better adapted to a mist environment? explain your answer

Explanation / Answer

In microscopic examination of eukaryotic microbes ( Algae – yeast)

1. What features did the cells you observed have in common ? how were they different?

Answer:

Common Features of Algae and Yeast:

1. Both are Eukaryotic organism

2. Both have true nucleaus with in the cytoplasm of cell

3. Both have the membrane bound cell organelles

4. Both are can unicellular

Difference in Algae and Yeast:

1. Algae contains photosynthetic structure but yeast is lacking.

2. Algae can be filamentous but yeast is non filamentous.

3. Algae can be munti-cellular but yeast is unicellular.

4. Algae can be autotroph but yeast is parasite or saprophyte.

What are some consequences of leaving a stain on a bacterial smear too long ( over staining) ?

Answer: When we leave the stain on a bacterial smear too long it may cause the disruption of cell wall and leads to cell lysis that results in the loss of true morphological characteristics of acterial cell.

What are some consequences of not leaving a staining on a smear long enough ( under-staining)

Due to under staining the bacterial cells may lose the stain or may not properly stained because when we washed the smear with alcohol or water it removes the stain and causes a problem in identifying the morphology of cell.

3. Consider a coccus and a rod of equal volume.

a. Which is more likely to survive in a dry environment? explain you answer

Answer: The Coccus is more likely to survive in a dry environment because coccus is circular in shape and occupy the minimum surface area as compared to baccilus with equal volume and does not allow to exchange of material (water) in the dry environment and survive longer.

b. Which would be better adapted to a moist environment? explain your answer

Answer: Bacillus or rod shaped bacteria would be better adapted to a moist environment becuase they have the better opportunity to absorb the nutrients from the environment due to higher surface area as compared to coccus or circular shaped bacteria.

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