Which of the following is the correct description of RT-PCR? A. Starting with mR

Question

Which of the following is the correct description of RT-PCR?

A. Starting with mRNA, we can create complementary DNA (cDNA).

B. Starting with double-stranded RNA, we can create complementary DNA (cDNA).

C. Starting with protein, we can create complementary DNA (cDNA).

D. None of the answer choices correctly describe RT-PCR.

E. Starting with tRNA, we can create complementary DNA (cDNA).

A. Starting with mRNA, we can create complementary DNA (cDNA).

B. Starting with double-stranded RNA, we can create complementary DNA (cDNA).

C. Starting with protein, we can create complementary DNA (cDNA).

D. None of the answer choices correctly describe RT-PCR.

E. Starting with tRNA, we can create complementary DNA (cDNA).

Explanation / Answer

Answer: Choice A

Explanation: Reverse transcriptase-PCR is a modified form of PCR in which the target mRNA is first converted into cDNA and this cDNA is then amplified using enzyme used in general PCR reaction. Hence, RT PCR is a very useful technique for those cells whose mRNA content is high but cDNA content is low.

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