5. (I point) Answer each of the following questions regarding base compositions.

Question

5. (I point) Answer each of the following questions regarding base compositions. a) State whether each of the following base composition relationships is true or false. i) A+ G = T + C ii) AT-G/C iv) A/C = G/T ii (A + C)(G+T-1.0 vi) A/G = T/C v) (A + T)/(C + G) = 1.0 b) The percentage of cytidine nucleotides in a double-stranded DNA segment is 17%. What is the percentage of adenosine in that DNA? c) A double-stranded DNA polynucleotide contains 80 thymidine and 110 guanosine nucleotides. What is the total number of base pairs in this DNA fragment? d) The percentage of adenosine nucleotides in a double-stranded DNA segment 500 base pairs long is 23%. How many cytidine nucleotides are present in this segment?

Explanation / Answer

Ans- (a) 1 true

2 true

3 false

4 false

5 true

6 true

Ans-B- the cytisine is 17% which means guinine is also 17% so the thymine and adenosine will be 100-34= 66% and when we want individual value of sdenosine it will be 66/2 = 33%

Ans-C-thymadine makes pair with adenosine and guanosine makes pair with cytosine so the total no of base pair will be 110+80= 190

Ans-D- total nucleotide =500

Adenosine = 23% or 23/100×500= 115

Adenosine makes pair with thymadine so thymadine will also be 115 and both makes a total of 230.

Cytosine and guanosine are left = 500-230= 270

They are present in equal number so the cytosine number will be 270/2= 135

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