QUESTION 3 Practically speaking, how would you determine that Nat influx into a

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QUESTION 3 Practically speaking, how would you determine that Nat influx into a cell underlies the early current? Replace intracellular Nat with its radioactive form and trace its movement across the membrane. Remove Na from the extracellular compartment and assess the early current under new conditions Use the voltage clamp method to measure the current. Treat the cell with tetraethylammonium QUESTION 4 Which process(es) underlie(s) the refractory period? The slow time course of turning off K conductance activation The persistence of Na conductance inactivation The slow time course of turning off K conductance activation and the persistence of Na* conductance inactivation The delay in K+ conductance activation QUESTION 5 2+ How would application of an intracellular Ca chelator affect the function of a synapse? It would increase the magnitude of postsynaptic potential It would eliminate the presynaptic Ca* current but have no effect on the postsynaptic potential It would increase the magnitude of presynaptic potential. 2+ It would eliminate the postsynaptic potential and Ca2+-dependent vesicle fusion.

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Answer:

1) Practically speaking, how would you determine that Na+ influx into a cell underlies the early current?

2) Which process underlies the refractory period?

3) How would application of an intracellular Ca2+ chelator affect the function of a synapse?

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