I AM ONLY REFERRING TO C PLEASE ONLY ANSWER C my guess of the answer is electron
ID: 190445 • Letter: I
Question
I AM ONLY REFERRING TO C PLEASE ONLY ANSWER C my guess of the answer is electron microscopy and in particularly. SEM, DIC microscopy, TEM. Please answer accurately according to question and I’ll instantly give a thumbs up thank you!2. Your friend shows you her data. She has been analyzing a protein (CB353) which is 576 amino acids long to determine which parts of the protein are responsible for its localization. She has created various deletion mutations in her protein and examined its localization, under normal conditions and also in the presence of leptomycin B, an inhibitor of the nuclear export machinery. Her data are depicted below. 9 marks total localization in the presence of eptomycin amino r acids nucleus cytoplasm nucleus cytoplasm 1-576 115-576 240-576 - 358-576- 240-357 15-357 1-114 15-239 From her data, answer the following questions: A. Which amino acids contain the nuclear localization signal (NLS)? Briefly explain. (3 marks) B. Which amino acids contain the nuclear export signal (NES)? Briefly explain. (3 marks) C. Describe a microscopic technique and procedure that your friend used to generate this data? (3 marks)
Explanation / Answer
C.
The data is generated using fluorescent microscopy.
Procedure:
Different cell lines harboring different constructs (GFP tagged) are illuminated with the UV light and the emitted light intensity is analyzed.
The intensity of the signal can also be quantified using flow cytometry (This does not tell about localization).
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