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Lab 3 Acids, Bases, Proteins and the pH scale Study Guide Use your text book, no

ID: 190583 • Letter: L

Question

Lab 3 Acids, Bases, Proteins and the pH scale Study Guide Use your text book, notes, and any of the websites from the lab assignment page in canvas to help you to answer the pre lab questions Pre Lab Questions: 1. Define the term Acid including their general properties. Provide a list of 3 common acids and their pH values you could find in your home 2. Define the term Base including their general properties. Provide a list of 3 common bases and their pH values you could find in your home. 3 In comparing hydrochloric acid (pH 1) and sodium hydroxide (pH 13) is one more dangerous than the other Why or why not? 4. Esplan the concept of protein denaturation List thaee wa)s that proteans can be denatured

Explanation / Answer

1.Acids are chemical compounds that can generate a proton ( hydrogen ion) in aqueous solutions, thus can act as electron pair acceptors.

They are sour in taste,can turn blue litmus paper to red, can react with bases to form salts, have a pH value below 7

Acetic acid is found in vinegar (5% Distilled white vinegar has a ph of aroun d 2.4-2.7)

citric acid is found in lemon and other citrous fruits (citric acid makes the pH of lemon juice around 2)

Lactic acid is found in milk (milk has a pH of around 6.5)

2. Bases are chemicals that generate hydroxide ion in aqueous solutions, or can accept a proton, thus acting as an electron pair donor.

they can turn red litmus blue, can react with acid to form salts, have a ph value above 7. Water soluble bases are called alkalis.

Lye soap which is used as oven cleaners has a pH of above 11 and is chemcially NaOH (sodium hydroxde)

Baking soda or sodium bi carbonate is basic in nature, has a pH of 8.1

washing soda or sodium carbonate is also basic in nature,has a pH of 11

3. Both are corrosive to our tissue at high concentrations.

HCl can form fumes and can cause respiratory distress as well as it reacts faster causing tissue burns, while NaOH is equally corrosive , the burns takes time to develop, and do not generate toxic fumes. Both react with water violently. But since HCl causes more and faster damage it can be considered more dangerous in comparison.

4. A protein is considered denatured when the hydrogen bonds, the salt bridges, the hydrophobic interaction, the disulfide bonds, all that hold the proper structure of a protein (which is responsible for its function) are distrupted rendering a distorted structure and a non functional protein.

Protein denaturation can be Chemical ( using chemcials like acids, alkalis, organic solvents like alcohols,ethers etc) Mechanical or physical (using blending, virgours shaking etc to denture protein) and thermal (using heat to denature proteins)