Why are cell types other than bacteria also used as hosts for recombinant DNA? S

Question

Why are cell types other than bacteria also used as hosts for recombinant DNA? Select the three correct answers. Some studies are carried out to research a specific DNA segment in a specific host environment. The mutation rate in bacteria is relatively high as compared to unicellular eukaryotes. Bacteria are not very easy to handle and grow in the lab. Bacteria do not process transcripts as do eukaryotes. Bacteria cannat car u sme postransdaional modifications as do eukaryotes. Bacteria possess an elaborate system of foreign DNA deactivation. Submit Request Answer

Explanation / Answer

Recombinant work is done in other hosts because, same segment of DNA may be transcribed and translated in a different way in different host systems, so if we want to study the gene expression or a promoter activation we need to have it done in a proper host which can provide a specific enviornment for the expression of that particular DNA.

Eukaryotic mRNA undergo post transcriptional modifications like 5'cappin, splicing, and polyAdenylation at 3' end. This does not happen in bacteria since they do not process the transcripts.

All types of post translational modifications like phosphorylation, methylation, acetylation,sumoylation etc may not be taking place in bacterial system as like in the host system.So inorder to get proper functional protein which has undergone every post translational modification, we need a proper host system that can provide that particular machinery.

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