10. For a new DNA strand, its synthesizing direction is: A Always 5\' to 3 B. Al
ID: 190664 • Letter: 1
Question
10. For a new DNA strand, its synthesizing direction is: A Always 5' to 3 B. Always 3' to5' C. Sometimes 5' to 3', sometimes 3' to 5' 11. For synthesizing DNA, which of the following material(s) is/are needed? A. dNTPs (A mixture of dATP, dTTP, dCTP, dGTP) B. Primer (a short stretch of polynucleotides) C. DNA polymerase D. DNA template E. All of above 12. Sanger's sequencing method is brilliant because he used ddNTPs to: A Continue the DNA strand synthesis when a ddNTP is added to the 5' phosphate group of previous nucleotide B. Continue the DNA strand synthesis when a ddNTP is added to the 3'-OH group of previous nucleotide C. Terminate the DNA strand synthesis when a ddNTP is added to the 5' phosphate group of previous nucleotide D. Terminate the DNA strand synthesis when a ddNTP is added to the 3'-0H group of previous nucleotide 13. Which of the following DNA polymerase is suitable for performing PCR? A. DNA polymerase I (from E. coli) B. Toq DNA polymerase (from Thermus aquoticus) C. Any DNA polymeraseExplanation / Answer
10) DNA polymerase can assemble DNA only in the 5' to 3' direction. The old strand is used as template for the assembly of a new DNA strand. The synthesis of a new strand begins with the synthesis of an RNA primer complementary to a pre-existing DNA strand.
Hence option (A) is correct.
11) For the synthesis of DNA starting material required taq DNA polymerase, primers, nucleotides and the buffer solution. DNA is made up of nitrogenious bases included pyrimidines (thymine and cytosine) and purines (adenine and guanine). The enzyme that replicate DNA is called as DNA polymerase. The old strand is used as template for the assembly of a new DNA strand.
Hence all the options are correct. Hence option (E) is correct.
12) Sangers sequencing is brillient because he used ddNTPs to added to 5' phosphate group of previous nucleotide. if we try to join the ddNTP to 3' OH group to 5' phosphate group of next nucleotide there is no enogh energy to weken the bond betweeen oxygen connected to 5' phosphorous group which has the nucleophilc attack.
Hence option (c) is correct.
13) Taq DNA polymerase is suitable for performing the PCR because it is obtained from the bacteria thermus aquaticus which lived in hot springs and hydrotheramal vents and this enzyme has ability to stand protein denaturating conditions at high temprature which required at the time of PCR.
Hence optiopn (B) is correct.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.