Cyanide affects the electron transport system by binding to components of the sy

Question

Cyanide affects the electron transport system by binding to components of the system (the cytochromes), thereby inhibiting the transfer of electrons. If seeds were treated with cya- nide, what results would they show in the tetrazolium test? Explain. 3. A farmer has a large bag of pea seeds left over from last year's planting. He would like to save some money by planting the seeds, but is not sure that a sufficient percentage of the seeds remains viable. How could he determine what percentage of the seeds would be expected to germinate? 4.

Explanation / Answer

3.

Tetrazolium test is used to distinguish between live and dead seeds.

It is a white powder which turns into red when reduced by enzymes present in the biologically active seeds.

If seeds are treated with cyanide, they will die. So, such seeds do not turn tetrazolium into red.

4.

a. He can do a sample germination assay. He can take 100 seeds and sow them in a small area and count the number of seedlings germinated.

b. He can also perform Tetrazolium test to identify the percentage of seed viability.

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