part a please 1. Suppose that someday a Martian bacterium is recovered. After tr
ID: 190959 • Letter: P
Question
part a please
1. Suppose that someday a Martian bacterium is recovered. After treatment with radiation, some colonies of this bacterium lose their characteristic green tinge (the mutant colonies are white). When wild type Martian bacterium (green-tinged) are broken open and the resulting cell extract is G used to treat the mutant (white) bacteria, some of the mutant bacteria reacquire the green tinge of wild type Martian bacteria To test the nature of Martian genetic material, separate portions of the cell extract are treated with DNase, RNase, proteinase K, and glycosidase, which are enzymes that hydrolyze DNA, RNA protein, and oligosaccharides, respectively. Cell extract treated with DNase and proteinase K could still cause reversion of the white Martian bacteria to green. Cell extract treated with either RNase or glycosidase was unable to cause reversion of white bacteria to green. a. What conclusions can be drawn from these experiments? Analysis of the lot of glycosidase used in the initial experiments revealed the presence of small amounts of proteins other than glycosidase. The treatment with glycosidase was repeated with RNasin included; RNasin is an inhibitor of RNase. b. What was the purpose of this last experiment? c. What would be your conclusion if the inclusion of RNasin in the glycosidase treatment restored the ability of the treated cell extract to cause reversion from white to green? d. What would be your conclusion if the cell extract incubated with glycosidase in the presence of asin stll was incapable of causing reversion from white to green bacteria? Be precise in your language. For example, DNA and RNA do not revert-bacteria do. I suggest that you make a diagram of the experiment similar in design to the diagrams on p. 50 of the packet You do not need to hand in this diagram; just use it to help your understanding of the experimentsExplanation / Answer
Background info:
Martian bacteria
Wild type: green
Mutant: white
Mutant treated with wild type(green) extract: Regain green color.
change of white to green
(DNA : NOT GENETIC MATERIAL)
change of white to green
(PROTEIN: NOT GENETIC MATERIAL)
no change of white to green
RNA: CAN BE THE GENETIC MATERIAL
no change of white to green
GLYCOSIDES: CAN BE THE GENETIC MATERIAL
This experiment provides an evidence on RNA or Glycosides as a genetic material in Martian bacteria.
Cell type treated with observation Wild type Extract DNAase(digests DNA)change of white to green
(DNA : NOT GENETIC MATERIAL)
WILD type Extract Proteinase K(digests protein)change of white to green
(PROTEIN: NOT GENETIC MATERIAL)
Wild type extract RNAase(Digests RNA)no change of white to green
RNA: CAN BE THE GENETIC MATERIAL
Wild type extract glycosidase(Digests gylcosides)no change of white to green
GLYCOSIDES: CAN BE THE GENETIC MATERIAL
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